Quasi-elastic light scattering of Artemia ribosomes sedimented in a CsCl density gradient
Identifieur interne : 004F42 ( Main/Exploration ); précédent : 004F41; suivant : 004F43Quasi-elastic light scattering of Artemia ribosomes sedimented in a CsCl density gradient
Auteurs : Paul Nieuwenhuysen [Belgique] ; Julius Clauwaert [Belgique]Source :
- Journal of Biochemical and Biophysical Methods [ 0165-022X ] ; 1981.
English descriptors
- Teeft :
- Autocorrelation function, Better resolution, Brine shrimp artemia, Centrifugation, Coefficient, Complete ribosomes, Correlation spectroscopy, Csci, Csci density gradient, Csci gradient, Density gradient, Diffusion coefficient, Good separation, Higher aggregates, Intensity profile, Large ribosome subunits, Large subunits, Laser, Laser beam, Laser light, Macromolecule, Methods enzymol, Molecular weight, Monodisperse solutions, Monomeric, Monomeric macromolecules, Next paragraph, Nieuwenhuysen, Photon, Photon correlation spectroscopy, Refractive index, Ribosomal subunits, Ribosome, Ribosome subunits, Room temperature, Sedimentation, Simple method, Spectroscopy, Standard conditions, Subunit, Sucrose, Sucrose gradient, Transparent centrifugation tube, Viscosity correction, Zonal centrifugation.
Abstract
Abstract: It is impossible to measure the diffusion coefficient of macromolecules directly and accurately by quasi—elastic light scattering, when aggregates cannot be eliminated from the solutions to be investigated. Nevertheless, a simple method can be applied to overcome this problem in many cases. Aggregates are separated from the monomeric macromolecules by rate-zonal sedimentation in a CsCl density gradient in a transparent centrifugation tube; the monomers are then located by laser light scattering intensity measurements; photon correlation spectroscopy of the scattered light finally yields their diffusion coefficient. The viscosity of aqueous CsCl solutions at different temperatures and concentrations allows a good separation by centrifugation and a low uncertainty in the reduction of the measured diffusion coefficient to standard conditions.The application of the method to eukaryotic large ribosomal subunits is described as an example.
Url:
DOI: 10.1016/0165-022X(81)90038-5
Affiliations:
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Nevertheless, a simple method can be applied to overcome this problem in many cases. Aggregates are separated from the monomeric macromolecules by rate-zonal sedimentation in a CsCl density gradient in a transparent centrifugation tube; the monomers are then located by laser light scattering intensity measurements; photon correlation spectroscopy of the scattered light finally yields their diffusion coefficient. 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